Cytocybernetics: Rapid and Reliable iPSC derived
Cardiac Myocyte Screening

Human iPSC Derived Cardiac Myocytes
Induced pluripotent stem cell derived cardiac myocytes hold great promise, but they
are limited in their use for studying integrated action potentials, as they lack IK1.
Our patented Cybercyte technology "electronically" expresses IK1, which restores the
physiological resting potential, and enables quantitative analysis of iPSCD action
potentials. Cells can be paced over a wide range of frequencies, and are not
confounded by spontaneous activity. Cells can clearly identified as atrial or
ventricular, which reduces variability and improves reliability.
Screening Services

Action Potential Services:
The effect of compounds on Human iPCS derived cardiac myocyte action potential
duration, restitution properties, EAD/DAD analysis, triangulation, upstroke velocity.
Cells are specifically ventricle or atrial, as needed. Custom protocols can be arranged.

Individual Channels :
Human iPSC derived cardiac myocytes can be used to determine the effect of a
compound on individual cardiac currents in the context of the human myocyte. The
Cybercyte is used to identify cells as ventricular or atrial, then channels are
assessed. Compounds can be tested against IKr, IKto, IKur, INa, ICa. Custom
protocols can be arranged. .

Call Cytocybernetics To discuss your specific requirements, at 1-855-351-6285, or
email us at
quotes@cytocybernetics.com.
Cytocybernetics: Connecting Cells and Computers
Cybernetics, your best choice for Human iPSC derived cardiac screening

The Team at Cytocybernetics has been working with the iPSCD cardiac myocytes, and
are the inventors of the Cybercyte which enables cells to identified as atrial or
ventricular, and makes iPSC derived myocytes suitable for rigorous quantitative
analysis.
A Sample of Human iPSC derived cardiac myocyte publications from the Team at
Cytocybernetics
For reprints, contact reprints@cytocybernetics.com

Electronic "expression" of the inward rectifier in cardiocytes derived from
human-induced pluripotent stem cells Bett GC, Kaplan AD, Lis A, Cimato TR,
Tzanakakis ES, Zhou Q, Morales MJ, Rssmusson RL. Heart Rhythm 2013 10:
1903-10.

Study familial hypertrophic cardiomyopathy using patient-specific induced pluripotent
stem cells Han L, Li Y, Tchao J, Kaplan AD, Lin B, Li Y, Mich-Basso J, Lis A, Hassan N,
London B, Bett Gc, Tobita K, Rasmusson RL, Yang L. Cardiovasc Res. 2014 104:
258-69.

Mechanism of automaticity in cardiomyocytes derived from human induced
pluripotent stem cells. Kim JJ, Yang L, Lin B, Zhu X, Sun B, Kaplan AD, Bett GC,
Rasmusson RL, London B, Salama G. J Mol Cell Cardiol. 2015 81: 81-93.

Reconciling computer models and stem cell models of human cardiac repolarization
Han L, Li T, Tchao J, Kaplan AD, Lin B, Li Y, Mich-Basso J, Lis A, Hassan N, London B,
Bett GC, Tobita K, Rasmusson RL, Yang L. Cardiovasc Res 2015 106:6-7.  

Modeling and study of the mechanism of dilated cardiomyopathy using induced
pluripotent stem cells derived from individuals with Duchenne muscular dystrophy.
Lin B, Li Y, Kaplan AD, Ao Y, Kalra S, Bett GC, Rasmusson RL, Denning C, Yang L,
Dis Model Mech 2015 8:457-66.