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VOLTAGE SENSITIVE DYES



Cytovolt1 (Di-4-ANBDQBS) and Cytovolt2 (Di-4-ANBDQPQ)


Order Dyes

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Publications



Cytovolt1 (Di-4-ANBDQBS) lasts up to four hours without damaging the cell.


Cytovolt2 (Di-4-ANBDQPQ) is for alternative applications.


Cytovolt1 Specifications

Our affordable, easy to use, single cell fluorescence recording system makes high quality optical measurements available to all. Our kit combines an optimized combination of voltage sensitive dye, excitation source, filter cube, and high sensitivity detector. This system works with our voltage sensitive dyes (Cytovolt1 (Di-4-ANBDQBS), Cytovolt2 (Di-4-ANBDQPQ)), and other dyes.



Our system features:

• Cytovolt1 (Di-4-ANBDQBS) and Cytovolt2 (Di-4-ANBDQPQ) long lasting voltage sensitive dyes

• Cytovolt Excite High-stability/low-noise excitation designed for Cytovolt1 (Di-4-ANBDQBS) and Cytovolt2 (Di-4-ANBDQPQ) voltage sensitive dyes.

• Cytovolt Cube Matched filter cube for our Cytovolt1 (Di-4-ANBDQBS) and Cytovolt2 (Di-4-ANBDQPQ) voltage sensitive dyes.

• Cytovolt Detector High-sensitivity/low-noise detector optimized for Cytovolt1 (Di-4-ANBDQBS) and Cytovolt2 (Di-4-ANBDQPQ) voltage sensitive dyes.



SELECT PUBLICATIONS USING OUR VOLTAGE SENSITIVE DYES, Cytovolt1 (Di-4-ANBDQBS) and Cytovolt2 (Di-4-ANBDQPQ)


S Iravanian, I Uzelac, AD Shah, MJ Toye, MS Lloyd, MA Burke, MA Daneshmand, TS Attia, JD Vega, M El-Chami FM Merchant, EM Cherry, NK Bhatia, FH Fenton (2023) Complex repolarization dynamics in ex vivo human ventricles are independent of the restitution properties Europace . 2023 Nov 2525(12):euad350. doi: 10.1093/europace/euad350
Credi C, Balducci V, Munagala U, Cianca C, Bigiarini S, de Vries AAF, Loew LM, Pavone FS, Cerbai E, Sartiani L, Sacconi L. Fast Optical Investigation of Cardiac Electrophysiology by Parallel Detection in Multiwell Plates. Front Physiol. 2021 Sep 312:692496.
I Martišienė, D Karčiauskas, A Navalinskas, R Mačianskienė, A Kučinskas, R Treinys, R Grigalevičiūtė, V Zigmantaitė, L Ralienė, R Benetis, J Jurevičius, Optical mapping of the pig heart in situ under artificial blood circulation Sci Rep. 2020 May 2210:8548. doi: 10.1038/s41598-020-65464-5
Lee P, Quintanilla JG, Alfonso-Almazán JM, Galán-Arriola C, Yan P, Sánchez-González J, Pérez-Castellano N, Pérez-Villacastín J, Ibañez B, Loew LM and Filgueiras-Rama D. In vivo ratiometric optical mapping enables high-resolution cardiac electrophysiology in pig models. Cardiovasc Res. 2019 Sep 1115(11):1659-1671.
Uzelac I, Crowley CJ, Fenton FH. Isosbestic Point in Optical Mapping Theoretical and Experimental Determination with Di-4-ANBDQPQ Transmembrane Voltage Sensitive Dye. Comput Cardiol (2010). 2019 Sep46:10.22489/CinC.2019.414. doi: 10.22489/CinC.2019.414. Epub 2020 Feb 24
A Klimas, G Ortiz, S Boggess, EW Miller, E Entcheva, Multimodal on-axis platform for all-optical electrophysiology with near-infrared probes in human stem-cell-derived cardiomyocytes Prog Biophys Mol Biol. 2019 Mar 5154:62–70. doi: 10.1016/j.pbiomolbio.2019.02.004
Streit J and Kleinlogel S. Dynamic all-optical drug screening on cardiac voltage-gated ion channels. Sci Rep. 2018 Jan 188(1):1153.
Shaheen N, Shiti A, Huber I, Shinnawi R, Arbel G, Gepstein A, Setter N, Goldfracht I, Gruber A, Chorna SV and Gepstein L. Human Induced Pluripotent Stem Cell-Derived Cardiac Cell Sheets Expressing Genetically Encoded Voltage Indicator for Pharmacological and Arrhythmia Studies. Stem Cell Reports. 2018 Jun 510(6):1879-1894
C Gloschat, K Aras, S Gupta, NR Faye, H Zhang, RA Syunyaev, RA Pryamonosov, J Rogers, MW Kay, IR Efimov. 2018. RHYTHM: An Open Source Imaging Toolkit for Cardiac Panoramic Optical Mapping. Scientific reports 8:2921.
K Thorsen, VS Dam, K Kjaer-Sorensen, LN Pedersen, VA Skeberdis, J Jurevicius, R Treinys, I Petersen, MS Nielsen, C Oxvig, JP Morth, VV Matchkov, C Aalkjaer, H Bundgaard, HK Jensen. 2017. Loss-of-activity-mutation in the cardiac chloride-bicarbonate exchanger AE3 causes short QT syndrome. Nature communications 8:1696.
A Klimas, CM Ambrosi, J Yu, JC Williams, H Bien, E Entcheva. 2016. OptoDyCE as an automated system for high-throughput all-optical dynamic cardiac electrophysiology. Nature communications 7:11542.
Wang K, Lee P, Mirams GR, Sarathchandra P, Borg TK,Gavaghan DJ, Kohl P, and Bollensdorff C. (2015). Cardiac tissue slices: preparation, handling, and successful optical mapping. Am J Physiol Heart Circ Physiol 308: H1112–H1125. Doi:10.1152/ajpheart.00556.2014.
BJ Hansen, J Zhao, TA Csepe, BT Moore, N Li, LA Jayne, A Kalyanasundaram, P Lim, A Bratasz, KA Powell, OP Simonetti, RS Higgins, A Kilic, PJ Mohler, PM Janssen, R Weiss, JD Hummel, VV Fedorov. 2015. Atrial fibrillation driven by micro-anatomic intramural re-entry revealed by simultaneous sub-epicardial and sub-endocardial optical mapping in explanted human hearts. Eur Heart J 36:2390-2401.
R Macianskiene, I Martisiene, A Navalinskas, R Vosyliute, R Treinys, B Vaidelyte, R Benetis, J Jurevicius. 2015. Evaluation of excitation propagation in the rabbit heart: optical mapping and transmural microelectrode recordings. PloS one 10:e0123050.
I Uzelac, Fenton, F.H.,. 2015. Robust Framework for Quantitative Analysis of Optical Mapping Signals without Filtering. Computing in Cardiology 42:461-464.
U Nussinovitch, L Gepstein. 2015. Optogenetics for in vivo cardiac pacing and resynchronization therapies. Nature biotechnology 33:750-754.
P Lee, P Yan, P Ewart, P Kohl, LM Loew, C Bollensdorff. 2012. Simultaneous measurement and modulation of multiple physiological parameters in the isolated heart using optical techniques. Pflugers Archiv : European journal of physiology 464:403-414.
P Lee, C Bollensdorff, TA Quinn, JP Wuskell, LM Loew, P Kohl. 2011. Single-sensor system for spatially resolved, continuous, and multiparametric optical mapping of cardiac tissue. Heart rhythm : the official journal of the Heart Rhythm Society 8:1482-1491.
RD Walton, D Benoist, CJ Hyatt, SH Gilbert, E White, O Bernus. 2010. Dual excitation wavelength epifluorescence imaging of transmural electrophysiological properties in intact hearts. Heart rhythm : the official journal of the Heart Rhythm Society 7:1843-1849.
M Warren, KW Spitzer, BW Steadman, TD Rees, P Venable, T Taylor, J Shibayama, P Yan, JP Wuskell, LM Loew, AV Zaitsev. 2010. High-precision recording of the action potential in isolated cardiomyocytes using the near-infrared fluorescent dye di-4-ANBDQBS. Am J Physiol Heart Circ Physiol 299:H1271-1281.
VV Fedorov, AV Glukhov, R Chang, G Kostecki, H Aferol, WJ Hucker, JP Wuskell, LM Loew, RB Schuessler, N Moazami, IR Efimov. 2010. Optical mapping of the isolated coronary-perfused human sinus node. J Am Coll Cardiol 56:1386-1394.
A Matiukas, BG Mitrea, M Qin, AM Pertsov, AG Shvedko, MD Warren, AV Zaitsev, JP Wuskell, MD Wei, J Watras, LM Loew. 2007. Near-infrared voltage-sensitive fluorescent dyes optimized for optical mapping in blood-perfused myocardium. Heart rhythm : The official Journal of the Heart Rhythm Society 4:1441-1451.


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